Volume No. :   3

Issue No. :  4

Year :  2013

ISSN Print :  2231-5705

ISSN Online :  2231-5713


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Delivery of Trypsin in to Cells using Nanoparticle



Address:   J. Madhusudhanan1*, P. Mohanambal2, V. Sowmya2
1Associate Professor and Head, Department of Biotechnology, Shri Andal Alagar College of Engineering, Mamandur 603 111.
1, 2Final Year, Department of Biotechnology, Shri Andal Alagar College of Engineering, Mamandur 603 111.
*Corresponding Author
DOI No:

ABSTRACT:
Nanoparticles are found to be useful as carrier for gene delivery. In the present study, the most biocompatible-Gold nanoparticles were used for carrying serine protease. i.e. Trypsin. It is sensitive to the protease inhibitors present inside the cells and these proteases on binding with gold nanoparticle, loses its stability but retain its activity. The Gold Nanoparticle along with the protease was characterized by UV-Vis spectroscopy, TEM and FTIR. The interaction was confirmed between Trypsin and Gold Nanoparticles and its size was found between 20-28nm. Further, the activity of Trypsin bound to Nanoparticles and the ability of AuNP’s as carrier were analyzed by invitro studies on E.coli HB101. Trypsin’s activity on intracellular protein and specifically on ALPase enzyme showed a 25-29% decrease in protein content which directs that Trypsin bound to Nanoparticles has found to be active within the cell. These results suggest that, such proteolytic enzyme can be designed with nanoparticle to lyse the defective proteins produced by the cells due to genetic disorder.
KEYWORDS:
Gold nanoparticles, Trypsin, Alkaline phosphatase, protein.
Cite:
J. Madhusudhanan, P. Mohanambal, V. Sowmya. Delivery of Trypsin in to Cells using Nanoparticle. Asian J. Pharm. Tech. 2013; Vol. 3: Issue 4, Pg 165-169.
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