Volume No. :   5

Issue No. :  2

Year :  2015

ISSN Print :  2231-5705

ISSN Online :  2231-5713


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Development and Validation of High Performance Liquid Chromatography Method for Levosulpiride and its Intermediate in Synthetic Mixture



Address:   Monika A. Rana*, Dr. Hasumati A. Raj
Department of Quality Assurance, Shree Dhanvantary Pharmacy College, Kim Surat, Gujarat India
*Corresponding Author
DOI No: 10.5958/2231-5713.2015.00015.X

ABSTRACT:
A simple, accurate and precise chromatography method was developed for Levosulpiride and 2-methoxy 5-sulfamoylbenzoyl benzoic acid methyl ester in synthetic mixture using RP-HPLC Method. In this chromatography method, both drug show its peak at 232 nm. Both the drugs show linearity in a concentration range of 10-50 µg/ml at their respective ?max. Optimize Mobile Phase was Water : Methanol : Acetonitrile (70 : 15 : 15). The relative standard deviation for accuracy, precision studies were found to be within the acceptance range (<2%). The limit of determination was 0.14µg/ml and 0.19µg/ml for Levosulpiride and 2-methoxy 5-sulfamoylbenzoyl benzoic acid methyl ester, respectively. The limit of quantification was 0.42µg/ml and 0.59µg/ml for Levosulpiride and 2-methoxy 5-sulfamoylbenzoyl benzoic acid methyl ester, respectively. Recovery of Levosulpiride and 2-methoxy 5-sulfamoylbenzoyl benzoic acid methyl ester were found to be 100.16 % and 99.15 % respectively confirming the accuracy of the proposed method. The proposed method is recommended for routine analysis since they are rapid, simple, accurate and also sensitive and specific by no heating and no organic solvent extraction.
KEYWORDS:
Levosulpiride, 2-methoxy 5-sulfamoylbenzoyl benzoic acid methyl ester, RP-HPLC method.
Cite:
Monika A. Rana, Hasumati A. Raj. Development and Validation of High Performance Liquid Chromatography Method for Levosulpiride and its Intermediate in Synthetic Mixture. Asian J. Pharm. Tech. 2015; Vol. 5: Issue 2, Pg 97-106.
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