Volume No. :   8

Issue No. :  3

Year :  2018

ISSN Print :  2231-5705

ISSN Online :  2231-5713


Registration

Allready Registrered
Click to Login

Development and Validation of RP-HPLC Method for Simultaneous Estimation of Saxagliptin and Dapagliflozin in Tablets



Address:   Sayali S. More, Sandeep S. Sonawane, Santosh S. Chhajed, Sanjay J. Kshirsagar
Department of Pharmaceutical Analysis, MET’s Institute of Pharmacy,
Bhujbal Knowledge City, Adgaon, Nashik–422 003
*Corresponding Author
DOI No: 10.5958/2231-5713.2018.00023.5

ABSTRACT:
A simple, accurate, precise and selective RP-HPLC method was developed and validated for simultaneous estimation of Saxagliptin (SAXA) and Dapagliflozin (DAPA) in tablet dosage form. Both drugs were separated on Phenomenex Hyperclone C18 column (250×4.6 mm, 5µ) using methanol: 20 mM phosphate buffer (pH3.0) (70:30, v/v) in an isocratic mode at flow rate of 1 mL/min. Chromatographic determination was carried out at wavelength of 225nm. SAXA and DAPA were eluted at 4.70 and 6.45min, respectively. The method was found linear in the range of 2-12 µg/mL and 4-24 µg/mL for SAXA and DAPA, respectively. The method was validated for linearity, accuracy, precision and selectivity. The developed method can be used for routine analysis of SAXA and DAPA tablets.
KEYWORDS:
Dapagliflozin, Saxagliptin, HPLC, Analytical Method Validation.
Cite:
Sayali S. More, Sandeep S. Sonawane, Santosh S. Chhajed, Sanjay J. Kshirsagar. Development and Validation of RP-HPLC Method for Simultaneous Estimation of Saxagliptin and Dapagliflozin in Tablets. Asian J. Pharm. Tech. 2018; 8 (3):145-148 .
[View HTML]     



Visitor's No. :   97416