Formulation of fast
dissolving tablets of Lisinopril using combination of
synthetic superdisintegrants
Rajeshree Panigrahi1*, K.A. Chowdary2, Gitanjali Mishra3, Manas Bhowmik4, Saiprasanna Behera1
1Royal College of Pharmacy and Health
Sciences - Berhampur
2St. Ann’s college of Pharmacy, Cantonment- Vizianagram
3Berhampur University- Berhampur
4Himalayan Institute of Pharmacy, Sikkim
*Corresponding
Author E-mail- ranuroyal@gmail.com
ABSTRACT:
Fast dissolving tablets of Lisinopril
were designed using combination of synthetic superdisintegrants
like croscarmellose sodium, crospovidone
and sodium starch glycolate in a ratio of 5:10 and
10:5 respectively by direct compression method. The blends of all formulations
were evaluated for various pre-formulation factors. Tablets were evaluated for
weight variation, hardness, disintegration time, drug content, friability and
in vitro dissolution. The formulation of Lisinopril
containing 10% crospovidone and 5% croscarmellose showed disintegration time of 145±0.502 secs
respectively with 99% drug release within 30 mins.
The results showed that super disintegrants used in
combinations shows better disintegrating property. The FTIR spectra showed no
interactions among them.
KEYWORDS: Fast Dissolving Tablet, Lisinopril,
croscarmellose sodium, crospovidone,
sodium starch glycolate
INTRODUCTION:
Recent advances in
novel drug delivery systems (NDDS) aim to enhance safety and efficacy of drug
molecule by formulating a convenient dosage form for administration and to
achieve better patient compliance. One such approach is fast dissolving tablets
1, 2. Lisinopril is a drug of the angiotensin converting enzyme (ACE) inhibitor class that is primarily used in treatment
of hypertension, congestive heart failure, heart attacks and also in preventing renal and retinal complications
of diabetes. The present study involved the comparison
between various synthetic superdisintegrants in
combination.
Materials and
methods:
Lisinopril
were obtained as a gift sample from Modern Lab., Indore (M.P). Croscarmellose sodium, crospovidone, sodium starch glycolate,
mannitol, magnesium stearate
were purchased from S.D. Fine Chemicals, India.
Preformulation Studies:
Angle of
repose:
Angle of repose (θ)
was determined using funnel method. The blend was poured through a funnel that
can be raised vertically until a maximum cone height (h) was obtained. The
radius of the heap (r) was measured and angle of repose was calculated3.
θ=
tan -1 (h/r)
Bulk density:
The term bulk density (b*) refers to a measure used to describe a
packing of particles. It is (gm/ml) and was determine using a balance and
measuring cylinder. Initially the weight of the measuring cylinder was tarred.
Then, drugs were poured into the measuring cylinder using a funnel and weighed
(M). Then volume of the powder (Vb) was taken. Bulk
density of the granules was calculated using following formula4.
b*
= M/Vb
Tapped
density:
Blend was tapped for a fixed number of taps. The minimum volume (Vt) occupied in the cylinder and
the weight (M) of the blend was measured. The tapped density (Electrolab) (t*) was calculated using following formula5.
t*
= M/ Vt
Hausner’s ratio:
Hausner’s
ratio is an index of ease of powder flow; it is calculated by following formula6.
Hausner
ratio = Tapped density/Bulk density
Carr’s Index
(CI) 11:
Tapped and bulk density measurements can be used to estimate the
Carr’s index of a material. Carr’s index was determined by,
C.I (%) = Tapped
density – bulk density /Tapped density*100
Formulation
of Fast Dissolving Tablets:
Fast dissolving tablets of Lisinopril
were prepared by direct compression method. The drug and excipients
were passed through sieve (#80) to ensure better mixing, synthetic superdisintegrants Croscarmellose
sodium, crospovidone, sodium starch glycolate were used in different proportions of 10:5 and
5:10 respectively. The powders were compressed into tablets on ten-station
rotary punch-tableting machine using 6 mm flat punch
set.
Evaluation of
Fast Dissolving Tablets:
Quality control tests for FDTs of all formulations were performed,
and the average values were calculated. All the tablets were evaluated for
different parameters as weight variation, hardness, friability, wetting time,
water absorption ratio, disintegration time and in vitro dissolution
study.
Weight
Variation:
Twenty tablets were selected randomly from each batch and weighed
individually on electronic balance (Shimadzu). The individual weighed is then
compared with average weight for the weight variations.
Hardness:
The strength of tablet is expressed as tensile strength (kg/cm2).
The tablet crushing load, which is the force required to break a tablet into
pieces by compression. It was measured using a tablet hardness tester (Monsanto
hardness tester). Three tablets from each formulation batch were tested
randomly and the average readings were noted7.
Friability:
Friability of the tablets was determined using Roche Friabilator. This device consists of a plastic chamber that
is set to revolve around 25 rpm for 4 min dropping the tablets at a distance of
6 inches with each revolution. Pre weighed sample of 20 tablets was placed in
the friabilator and were subjected to 100
revolutions. Tablets were dusted using a soft muslin cloth and reweighed. The
friability (F %) is given by the formula8
F (%) = (1 – W0 / W) × 100
Where, W is weight of the
tablets before the test
W0
is the weight of the tablets after test.
Wetting time:
Five circular tissue papers of 10 cm diameter were placed in a
Petri dish with a 10cm diameter. 10 ml of water at 37±0.50 containing eosin, a
water-soluble dye, was added to the petridish. A
tablet was carefully placed on the surface of tissue paper. The time required
for water to reach the upper surface of the tablets was noted as the wetting
time. Six tablets from each formulation batch were tested randomly and the
average reading noted9.
Water
absorption ratio:
A piece of tissue paper folded twice was placed in a small petridish containing 6 ml of water. A tablet was put on the
paper and the time required for complete wetting was measured. The wetted
tablet was then weighed. Water absorption ratio R, was determined using
following equation,
R = Wa – W b/ Wb × 100
Where Wa = weight of tablet after absorption
Wb
= weight of tablet before absorption
In vitro disintegration time:
Disintegration
time was measured using a disintegration apparatus. Tablets were added to 10 ml of Sorenson’s
buffer solution of pH 6.8 at 37 ± 0.5°C. Time required for disintegration of
the tablets was noted.
In vitro drug
release:
In vitro drug release of Lisinopril
fast dissolving tablets was determined using USP Dissolution Apparatus II. The
dissolution test was performed using 900 ml of Phosphate buffer (6.8) at 37 ±
0.50. The speed of rotation of paddle was set at 50 rpm. 5 ml samples were
withdrawn at time points of 5, 10, 15, 20, 25, and 30 min and same volume was
replaced with fresh media. Absorbance of solution was checked by UV
spectrophotometer at a wavelength of 206 nm10.
Figure 1. Cumulative % drug release of Lisinopril FDT in phosphate buffer PH 6.8 using synthetic superdisintegrants in combination
Figure
2. Log % drug retained for Lisinopril FDT in phosphate buffer PH 6.8 using synthetic superdisintegrants in combination
Table
1. Formulation of Lisinopril
FDT using combination of synthetic superdisintegrants:
Ingredient |
CP-CCS 1L1 |
CP-CCS 2L2 |
CP-SSG 1L3 |
CP-SSG 2L4 |
CCS-SSG
1L5 |
CCS-SSG
2L6 |
Lisinopril |
30 |
30 |
30 |
30 |
30 |
30 |
MCC |
20 |
20 |
20 |
20 |
20 |
20 |
CPV |
20 |
10 |
20 |
10 |
- |
- |
CCS |
10 |
20 |
- |
- |
20 |
10 |
SSG |
- |
- |
10 |
20 |
10 |
20 |
Mannitol |
116 |
116 |
116 |
116 |
116 |
116 |
Mg.st |
2 |
2 |
2 |
2 |
2 |
2 |
Talc |
2 |
2 |
2 |
2 |
2 |
2 |
Total |
200 |
200 |
200 |
200 |
200 |
200 |
Table
2. Evaluation of Lisinopril
FDT using combination of synthetic superdisintegrants:
Formulation |
L1 |
L2 |
L3 |
L4 |
L5 |
L6 |
Hardness |
3.1±0.0082 |
2.8±0.057 |
2.9±0.066 |
2.8±0.05 |
2.8±0.052 |
2.7±0.055 |
DT |
145±0.502 |
152±0.235 |
167±0.787 |
172±1.003 |
176±0.047 |
179 ±0.996 |
Friability |
0.013 ±
0.0004 |
0.014
±0.0001 |
0.014
±0.0001 |
0.015
±0.0006 |
0.016
±0.0003 |
0.017
±0.0004 |
Wetting
time |
21±0.656 |
26±0.661 |
25±0.771 |
29±0.694 |
27±0.521 |
31±0.539 |
Dispersion
time |
25±0.773 |
29±0.702 |
28±0.683 |
31±0.541 |
32±0.64 |
35±0.586 |
Water
abs ratio |
78±1.352 |
64±0.646 |
72±1.125 |
60±0.623 |
54±0.7346 |
49±0.669 |
Table
3. Preformulation
studies of Lisinopril FDT using combination of
synthetic superdisintegrants:
Formulation |
L1 |
L2 |
L3 |
L4 |
L5 |
L6 |
Bulk
density |
0.52±0.005 |
0.53±0.0053 |
0.52±0.0052 |
0.53±0.0055 |
0.54±0.005 |
0.54±0.005 |
Tapped
density |
0.54±0.005 |
0.55±0.0053 |
0.55±0.0054 |
0.56±0.0059 |
0.56±0.0058 |
0.58±0.006 |
Hausner’s ratio |
1.03±0.195 |
1.03±0.196 |
1.05±0.001 |
1.05±0.001 |
1.03±0.0005 |
1.07±0.0005 |
Carr’s
index |
3.70±0.007 |
3.63±0.006 |
5.45±0.007 |
5.35±0.0065 |
3.57±0.1 |
6.89±0.16 |
Angle
of Repose |
31±0.55 |
32±0.57 |
31±0.554 |
33±0.58 |
32.5±0.59 |
33±0.586 |
Table
4. Cumulative % drug release of Lisinopril FDT in phosphate buffer PH 6.8 using synthetic superdisintegrants in combination:
TIME IN MINUTES |
L1 |
L2 |
L3 |
L4 |
L5 |
L6 |
2 |
32.09 |
38.12 |
21.98 |
41.78 |
16.98 |
12.89 |
4 |
47.19 |
49.98 |
21.99 |
41.78 |
22.79 |
12.9 |
6 |
55.21 |
51.23 |
47.32 |
49.23 |
37.77 |
25.76 |
8 |
72.37 |
54.23 |
59.65 |
51.13 |
43.95 |
44.34 |
10 |
85.85 |
78.12 |
79.65 |
61.87 |
55.49 |
47.76 |
15 |
90.98 |
89.98 |
92.23 |
98.99 |
67.87 |
56.87 |
20 |
99.89 |
99.99 |
99.97 |
98.99 |
99.97 |
97.98 |
25 |
99.89 |
-- |
--- |
-- |
-- |
99.98 |
Table
5. Log % drug retained for Lisinopril FDT in phosphate buffer PH 6.8 using synthetic superdisintegrants in combination:
TIME IN MINUTES |
L1 |
L2 |
L3 |
L4 |
L5 |
L6 |
2 |
1.83193373 |
1.79155031 |
1.8922059 |
1.7650722 |
1.91918273 |
1.940068 |
4 |
1.72271617 |
1.69914369 |
1.8921503 |
1.7650722 |
1.88767355 |
1.9400182 |
6 |
1.65118106 |
1.68815276 |
1.7216458 |
1.70560716 |
1.7939998 |
1.870638 |
8 |
1.44138088 |
1.66058091 |
1.6058435 |
1.68904234 |
1.74857562 |
1.7455432 |
10 |
1.15075644 |
1.34004732 |
1.3085644 |
1.58126681 |
1.64845759 |
1.7180032 |
15 |
0.95520654 |
1.00086772 |
0.890421 |
0.00432137 |
1.50691073 |
1.6347795 |
20 |
-0.95860731 |
-2 |
-1.522879 |
0.00432137 |
-1.5228787 |
0.3053514 |
25 |
-0.95860731 |
-- |
-- |
-- |
-- |
-- |
a.
Optimized formulation (L1)
Figure 3. FTIR Spectra
RESULTS AND DISCUSSION:
The formulation and results for evaluation
of different batches of Lisinopril FDT’s prepared by
direct compression method are shown in Table 1 and 2. The most important
parameter that needs to be optimized in the development of Fast Dissolving
tablets is the disintegration time of tablets. In the present study tablets in
all the batches disintegrated in the range of 140 –180 secs
fulfilling the official requirements for Fast Dissolving tablets. It was
observed that the formulations (L1 – L6) containing combinations of synthetic superdisintegrants used in different ratios of 10:5 and
5:10 respectively were prepared. The formulations contained crospovidone
(CP), croscarmellose (CCS) and sodium starch glycolate (SSG) in combined form as CP and CCS in
combination of 10:5 and 5:10 ratios, CP and SSG in combination of 10:5 and 5:10
ratios and CCS and SSG in combination of 10:5 and 5:10 ratios respectively. The
tablet formulations containing 10:5 ratios viz. 20 mg of CP and 10 mg of CCS
(L1), 20 mg of CP and 10 mg of SSG (L3), 20 mg of SSG and 10 mg of CCS (L6)
showed DT as 145, 167 and 179 secs and formulations
containing 5:10 ratios viz. 10 mg of CP and 20 mg of CCS (L2), 10 mg of SSG and
20 mg of CP (L4), 10 mg of SSG and 20 mg of CCS (L5), showed DT as 152, 172,
176 secs respectively. The wetting time is important
to check minimum volume of water required for wetting of the tablet. The
wetting time for formulations L1 –L6 showed in the range of 21 to 31 secs respectively. It has been reported that wetting is
closely related to the inner structure of the tablets and the hydrophillicity excipients. The superdisintegrants show its disintegrant
effect by swelling action. Thus the result indicates that these tablets would
disintegrate almost instantaneously when they will come in contact with even
slight amount of saliva in the mouth. The Preformulation
studies were carried out and all the parameters passed the requirements for all
formulations as shown in Table 3. The cumulative percentage drug release of the
tablets from the prepared batches was found to be 99.99% due to the effect of
two superdisintegrants in combination as shown in
Table 4 and 5 respectively. It was observed that nearly all the batches showed
drug release close to 100% in phosphate buffer pH 6.8. The in-vitro drug
release for the formulations L1 –L6 was found to be in the range of 98.99 -99.99%
respectively. The FTIR studies were carried out for the pure drug Lisinopril, superdisintegrants
and the optimized formulation L1 and the spectra’s showed no interaction. FT-IR
spectra of pure drug Lisinopril (Figure-3.a) revealed the presence of characteristic peak of N-H
stretching around 3557.85cm-1, O-H stretching around 3300 cm-1,
aromatic C-H stretching around 3200cm-1, sp3 C-H
stretching at 2957cm-1, C=O stretching around 1700 cm-1
and C-O stretching around 1045 cm-1. The FT-IR spectra of croscarmellose sodium
revealed the presence of characteristic broad and strong peak for OH
stretching around 3413.19 cm-1, stretching peak around 2891.42 cm-1
corresponds to the stretching vibration of CH3 group, a strong
band around 1600cm-1 corresponds to the carbonyl stretching and C-O
stretching around 1166cm-1. (Figure-3.b).
FT-IR spectra of crospovidone revealed the presence
of characteristic peak for CH3 group at 2991.72cm-1, C=O
stretching peak at 1615cm-1. Peaks in the region of 1400 cm-1
indicate the stretching vibration of C-C. Peak at 843.89 cm-1
indicates presence of methylene out of plane bending
(Figure–3.c). FT-IR spectra sodium starch glycolate revealed the presence of characteristic peaks
of OH around 3472cm-1, stretching peak around 2969.54cm-1 corresponds
to CH3, peaks around 1663cm-1 correspond to stretching of
C=O and C-O stretching around 1163cm-1.
(Figure -3.d). FT-IR spectra of the optimized
formulation (L1) revealed the presence of
characteristic peaks of N-H stretching, O-H stretching, aromatic C-H
stretching, sp3 C-H stretching, C=O stretching and C-O stretching (Figure -3.e ). The formulation L1 consists of crospovidone 20 mg and croscarmellose
10 mg which improved the DT to an extent as both the superdisintegrants
are ranked to be better in terms of swelling and disintegrating the tablets.
CONCLUSION:
Fast Dissolving tablets of Lisinopril
were prepared by direct compression method using crospovidone
(CP), croscarmellose (CCS) and sodium starch glycolate (SSG) in combined form as CP and CCS in
combination of 10:5 and 5:10 ratios, CP and SSG in combination of 10:5 and 5:10
ratios and CCS and SSG in combination of 10:5 and 5:10 ratios respectively.
From the observed parameters it was concluded that the formulation (L1)
satisfied all the official requirements. The tablets had acceptable hardness of
average 3.1 kg/ cm2, 0.013 % friability, In-vitro disintegration time 145 secs
and in-vitro drug release of
99.98%. Hence it can be concluded that using a combination of synthetic superdisintegrants would be quite effective in providing
faster onset of action without the need of water for swallowing.
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Received on 16.06.2012 Accepted
on 16.07.2012
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Asian J. Pharm. Tech. 2(3): July-Sept. 2012; Page 94-98