Quantitative Evaluation of Carbohydrate Levels in Fruits by UV-Visible Spectrophotometer

 

Vavilala Prateesh Kumar*, Ch. Madhu*, Kavya Mannem, V.S. Asha, A. Sambasiva Rao, M. Sravan Prasad

Sri Indu Institute of Pharmacy, Sheriguda, R.R dist.501510

*Corresponding Author E-mail-prateesh.vavilala@gmail.com

 

ABSTRACT:

A rapid method was developed for the quantitative estimation of carbohydrates present in the different natural fruits by UV-Visible Spectrophotometer. The sample extract of the apple, banana, custard apple, guava, papaya, grapes, pineapple, orange, pomegranate, kiwi fruit, mango, strawberries were subjected by using anthrone as reagent for the quantitative estimation of the carbohydrate. These samples absorbance was read in UV-Visible spectrophotometer at the wavelength of 750nm. The recommendation for the general population is that carbohydrate should supply 50 to 55 percent of total calories, and 130 grams per day (520 calories per day) for male and female adults and for athletes is between 55 and 65 percent of total calories.

 

KEYWORDS: Fruits, UV-Visible spectrophotometer, wavelength, anthrone, absorbance

 


INTRODUCTION:

Carbohydrates act as the primary source of energy which is converted into glucose to generate energy essential for metabolism in every cell of the body1. Though there is no absolute requirement of carbohydrates, they are essential to ensure that energy is available to the body to perform its normal functions. Carbohydrates perform numerous roles in living things. Polysaccharides serve for the storage of energy (e.g., starch and glycogen), and as structural components (e.g., cellulose in plants and chitin in arthropods)1-5. The 5- carbon monosaccharide ribose is an important component of coenzymes (e.g., ATP, FAD, and NAD) and the backbone of the genetic molecule known as RNA. The related deoxyribose is a component of DNA(2). Saccharides and their derivatives include many other important biomolecules that play key roles in the immune system, fertilization, preventing pathogenesis, blood clotting, and development.

 

In food science and in many informal contexts, the term carbohydrate often means any food that is particularly rich in the complex carbohydrate starch (such as cereals, bread, and pasta) or simple carbohydrates, such as sugar (found in candy, jams, and desserts) For most people, between 40% and 60% of total calories should come from carbohydrates, preferably from complex carbohydrates (starches) and naturally occurring sugars4. Complex carbohydrates provide calories, vitamins, minerals, and fiber. Carbohydrates formula is C12H22O11.

 

EXPERIMENTAL:

Chemicals and Reagents:

Anthrone, Sulphuric acid were purchased from Merck Specialties Pvt. Ltd.

 

Instrumentation:

Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflectance spectroscopy in the ultraviolet-visible spectral region. This means it uses light in the visible and adjacent (near-UV and near-infrared (NIR)) ranges. The absorption or reflectance in the visible range directly affects the perceived color of the chemicals involved. In this region of the electromagnetic spectrum, molecules undergo electronic transitions.

 

 

Sample preparation:

Apple, banana, custard apple, guava, papaya, grapes, pineapple, orange, pomegranate, kiwi fruit, mango, strawberries were taken as samples. 5 grams of each sample was extracted separately in 25ml of distilled water3.

 

Reagent Preparation:

0.2 grams of anthrone was weighed accurately and dissolved in concentrated sulphuric acid and make up the volume up to 100ml and finally transferred it in to a 100ml of reagent bottle.5

 

Procedure:

Pipette out the 1ml of each extracted sample in to a 25ml of volumetric flask and add 2ml of freshly prepared anthrone reagent in each volumetric flask and finally make up the volume up to the mark with distilled water. Reference was prepared by taking 2ml of anthrone reagent in a 25ml of volumetric flask and make up the volume up to the mark with distilled water. To the above prepared samples wavelength was check in UV-visible spectrophotometry and wavelength was set at 750nm at that wavelength the developed colour absorbances were noted for the above mentioned samples5.

 

RESULT AND DISCUSSION:

From the experiment in apple 1.255%, banana 1.35%, custard apple 1.26%, guava 0.55%, papaya 0.685%, grapes 0.79%, pineapple 1.015, orange 0.77%, pomegranate 1.3%, kiwi fruit 0.6%, mango 1.75%, strawberries 0.4% of carbohydrates are evaluated. The recommendation for the general population is that carbohydrate should supply 50 to 55 percent of total calories, and 130 grams per day (520 calories per day) for male and female adults and for athletes is between 55 and 65 percent of total calories.

 

Table 1:

Food Amount of Carbohydrate

Apple 1.255%

Banana 1.35%

Custard apple 1.26%

Guava 0.55%

Papaya 0.685%

Grapes 0.79%

Pineapple 1.015%

Orange 0.77%

Pomegranate 1.3%

Kiwi fruit 0.6%

Mango 1.75%

Strawberries 0.4%

 

 

CONCLUSION:

From the Mango we gain high calories of carbohydrates of total calories to increase the activity levels in the body.

 

REFERENCES

1. Flitsch SL and Ulijn RV. Sugars tied to the spot. Nature. 2003;421:219220.

2. Maton Anthea, Jean Hopkins, Charles William McLaughlin, Susan Johnson, Maryanna Quon Warner, David LaHart and Jill D. Wright. Human Biology and Health. Englewood Cliffs, New Jersey, USA: Prentice Hall. 1993:5259.

3. John Merle Coulter, Charler Reid Barnes and Henry Chandler Cowles. A Textbook of Botany for Colleges and Universities. 1930.

4. Carl A. Burtis, Edward R. Ashwood and Norbert W. Tietz. Tietz Fundamentals of Clinical Chemistry.2000.

5. Matthews C E, Van Holde KE and Ahern KG. Biochemistry. 3rd edition. Benjamin Cummings. 1999.

 

 

 

 

Received on 29.06.2012 Accepted on 16.07.2012

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Asian J. Pharm. Tech. 2(3): July-Sept. 2012; Page 99-100