Author(s):
Satish Shelke, Nital Ingle, Ashish Tale, Balaji Thakare, Rahul Kalwe, Shirish Jain
Email(s):
balaji.thakare2@gmail.com , shelkesp21@gmail.com , jayshree.thakare33@gmail.com , ashishtale93@gmail.com , rahulkalwe20@gmail.com , ashishtale93@gmail.com
DOI:
10.52711/2231-5713.2024.00048 
Address:
Satish Shelke1, Nital Ingle1, Ashish Tale1, Balaji Thakare2*, Rahul Kalwe2, Shirish Jain1
1Department of Quality Assurance, Rajarshi Shahu College of Pharmacy, Buldhana 443001 (M.S.), India.
2Anuradha College of Pharmacy, Chikhali, Dist. Buldana (M.S.), India.
*Corresponding Author
Published In:
Volume - 14,
Issue - 4,
Year - 2024
ABSTRACT:
A combination of Metformin and Evogliptin is used to treat type 2 diabetes mellitus. A selective, accurate and precise RPHPLC method was developed and validated for simultaneous estimation of these drugs in bulk and tablet dosage forms. The drugs were resolved on a C18 (150mm × 4.6mm, 5µ) column using Acetonitrile: water (75:25 v/v) as the mobile phase. The detection wavelength was 226 nm. The retention times obtained for metformin and evogliptin were 1.187 and 2.273 min respectively. The linearity ranges were 250-1250 and 2.5-12.5 µg/ml respectively with Regression coefficients of 0.9974 and 0.9994. The % RSD of precision studies was found to be less than 2%. The Accuracy of the proposed method was determined by recovery studies and the mean recovery was found to be in between 98-102%. The method was also applicable for quantitative analyses of the marketed tablet formulations and in studying stability of the drugs under acidic, alkaline, oxidation, thermal and UV conditions.
Cite this article:
Satish Shelke, Nital Ingle, Ashish Tale, Balaji Thakare, Rahul Kalwe, Shirish Jain. Development and Validation of Stability Indicating RP-HPLC Method for Evogliptin and Metformin in Bulk and Tablets. Asian Journal of Pharmacy and Technology. 2024; 14(4):299-6. doi: 10.52711/2231-5713.2024.00048
Cite(Electronic):
Satish Shelke, Nital Ingle, Ashish Tale, Balaji Thakare, Rahul Kalwe, Shirish Jain. Development and Validation of Stability Indicating RP-HPLC Method for Evogliptin and Metformin in Bulk and Tablets. Asian Journal of Pharmacy and Technology. 2024; 14(4):299-6. doi: 10.52711/2231-5713.2024.00048 Available on: https://ajptonline.com/AbstractView.aspx?PID=2024-14-4-1
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