Author(s):
Shubham Porte, Vinayak Kaushik, Geetanjali Sahu, Sharang Bali
Email(s):
shubham.sp.porte@gmail.com , iamvinayak49@gmail.com , geetanjali.sahu574@gmail.com
DOI:
10.52711/2231-5713.2025.00050 
Address:
Shubham Porte1, Vinayak Kaushik1, Geetanjali Sahu2, Sharang Bali2
1M. Pharma, School of Pharmacy, Chouksey Engineering College, Lalkhadan, Bilaspur - 495004 Chhattisgarh.
2Assistant Professor, School of Pharmacy, Chouksey Engineering College, Lalkhadan, Bilaspur - 495004 Chhattisgarh.
*Corresponding Author
Published In:
Volume - 15,
Issue - 4,
Year - 2025
ABSTRACT:
Hibiscus rosa sinensis a widely known ornamental and medicinal plant has the history of traditional medicinal uses throughout the world, and is still used in many ways due to diverse medicinal properties, this plant has also been used as a showy flower. Flavonoids, Alkaloids, Anthracyanins Tannins Saponins and other phytochemicals have shown significant therapeutic effects such as antioxidants, anti-inflammatory, antimicrobial and wound healing. This experiment is focuses on the extraction of these phytochemical compounds and preliminary phytochemical screening (Identification test) from Hibiscus rosa sinensis flower with the ethanol as the solvent running through Soxhlet apparatus, dried flower powder was subjected to continuous hot percolation method up to 48hours at the control temperature in between 70-80°C. The extraction method was proven to be highly effective in extracting the thermo stable phytochemicals, the final extract was evaporated and dried, test was performed such as Shinoda test, ferric chloride test and Wagner’s test to confirm the presence of certain phytochemical groups. These findings solidify the therapeutic properties and potential for future studies in the Hibiscus rosa sinensis. This experiment contributes to the understanding of the plants extraction process, chemical profile, medicinal properties and possible use advance drug delivery systems as the Phytopharmaceutical product.
Cite this article:
Shubham Porte, Vinayak Kaushik, Geetanjali Sahu, Sharang Bali. Extraction of Hibiscus rosa sinensis Flower. Asian Journal of Pharmacy and Technology. 2025; 15(4):339-5. doi: 10.52711/2231-5713.2025.00050
Cite(Electronic):
Shubham Porte, Vinayak Kaushik, Geetanjali Sahu, Sharang Bali. Extraction of Hibiscus rosa sinensis Flower. Asian Journal of Pharmacy and Technology. 2025; 15(4):339-5. doi: 10.52711/2231-5713.2025.00050 Available on: https://ajptonline.com/AbstractView.aspx?PID=2025-15-4-3
REFERENCES:
1. Amminbavi D, Lakshmi NP. Assessment of in vitro wound healing potential of Hibiscus leaf extract emulgel. Asian J Pharm Res. 2020; 10(2): 67-72.
2. Devgun M, Nanda A, Ansari SH, Swamy SK. Recent techniques for extraction of natural products. Res J Pharm Technol. 2010;3(3):644-9.
3. Pawar AR, Vikhe DN, Jadhav RS. Recent advances in extraction techniques of herbals – a review. Asian J Res Pharm Sci. 2020; 10(4): 287-92.
4. Jadhav VM, Thorat RM, Kadam VJ, Sathe NS. Hibiscus rosa sinensis Linn ‘‘Rudrapuspa’’: a review. J Pharm Res. 2009; 2(7): 1168-73.
5. Khristi V, Patel VH. Therapeutic potential of Hibiscus rosa sinensis: a review. Int J Nutr Diet. 2016; 4(2): 105-23.
6. Chakraborty A, Bhattacharjee A, Chakraborty M, Mukhopadhyay G. Standardization of Hibiscus rosa-sinensis flower by HPTLC, HPLC and AAS. Res J Pharm Technol. 2023; 16(12): 5657-62.
7. Patil RM, Shaikh AZ, Patil DR, Jain AS, Shaikh SR, Pawar SP. A short review on effective extraction procedures for herbal drugs. Res J Pharmacogn Phytochem. 2024; 16(4): 249-53.
8. Ashraf M, Kumar RM. Hibiscus rosa-sinensis in the spotlight: a critical review on the bioactivity and drug development potential. Int J Pharm Sci. 2025; 3(6): 4431-42.
9. Mejía JJ, et al. Color, antioxidant capacity and flavonoid composition in Hibiscus rosa-sinensis cultivars. Molecules. 2023; 28(4): 1779. doi:10.3390/molecules28041779.
10. Amtaghri S, et al. A comprehensive overview of Hibiscus rosa-sinensis L.: ethnobotanical uses, phytochemistry, therapeutic uses, pharmacological activities, and toxicology. Endocr Metab Immune Disord Drug Targets. 2024; 24(1): 86-115. doi:10.2174/1871530323666230522113405.
11. Harini VS. Evaluation of the anticancer, antidiabetic, and in vitro wound healing properties of aqueous and ethanolic extract of Hibiscus rosa-sinensis L. J Pharm Bioallied Sci. 2024;16(Suppl 2): S1217-22. doi: 10.4103/jpbs.jpbs_545_23.
12. Jamrozik D, et al. Hibiscus sabdariffa in diabetes prevention and treatment: does it work? An evidence-based review. Foods. 2022; 11(14): 2134. doi:10.3390/foods11142134.
13. Irawan B, Azhary M, Sary NL. In silico studies of bioactive compounds of Hibiscus rosa-sinensis against IL-8 and LTB-4 in chronic obstructive pulmonary disease. Res J Pharm Technol. 2025; 18(6): 2700-7.
14. Banu Z, Qhursheed A, Alekya A, Shirisha B, Mounika BV, Divya B. Phytochemical and pharmacological screening of Cosmos sulphureus, Ruellia simplex and Hibiscus rosa sinensis flower extracts for antinociceptive activity. Res J Pharm Technol. 2024; 17(7): 3399-404.
15. Rakesh P, Sujit P, Janeshwer Y. Isolation and identification of compounds from the leaves extract of Hibiscus syriacus L. Asian J Pharm Technol. 2015; 5(1): 8-12.
16. Nevede SA, Lokapure SG, Kalyane NV. Study on anti-solar activity of ethanolic extract of Hibiscus rosa-sinensis Linn flower. Res J Pharm Technol. 2011; 4(3): 472-3.
17. Sarwade PP, Bongale MM, Mittal N, Chand S, Vijayalakshmi K, Khongshei RR, et al. A detailed study of Hibiscus rosa-sinensis L.: phytochemistry, pharmacological activities, therapeutic uses and antimicrobial, antioxidant activities. Asian J Pharm Res Dev. 2025; 13(1): 138-46. doi:10.22270/ajprd. v13i1.151.
18. Shelke M, Parjane S, Mankar SD, Siddheshwar SS. Therapeutic potential of Hibiscus rosa sinensis – a review. Res J Sci Technol. 2021; 13(2): 151-6.
19. Khan IM, Rahman R, Mushtaq A, Rezgui M. Hibiscus rosa-sinensis L. (Malvaceae): distribution, chemistry and uses. Int J Chem Biochem Sci. 2017; 12: 147-51.
20. Magdalita PM, San Pascual AO. Hibiscus (Hibiscus rosa-sinensis): importance and classification. In: Floriculture and ornamental plants. Singapore: Springer; 2022. p. 483-522.
21. Vélez-Gavilán J. Hibiscus rosa-sinensis (Chinese rose). Forest. 2006.
22. Bahuguna A, Vijayalaxmi KG, Suvarna VC. Formulation and evaluation of fresh red Hawaiian hibiscus (Hibiscus rosa-sinensis) incorporated value added products. Int J Curr Microbiol Appl Sci. 2018; 7(8): 4282-90.
23. Punasiya R, Pillai S. In vitro antibacterial activity of leaf extracts of Hibiscus syriacus (L). Res J Top Cosmet Sci. 2014; 5(2): 51-5.
24. Yuan Y, Sun J, Guo Y, Zhao X, Zhu L. Comprehensive profiling of anthocyanins in sixteen cultivars of Hibiscus rosa-sinensis L. Molecules. 2023; 28(4): 1779.
25. Rajendran R, Balasubramanian S. Phytochemical profiling and morphological characterization of Hibiscus rosa-sinensis and Clitoria ternatea flower petal extracts. Biomed Pharmacol J. 2024; 18(2): 1231-40.
26. Dávila I, Medina-Mendoza A, Ariza-Ortega N, Godínez-Ramírez L. Solvent selection for phenolic compounds extraction from Hibiscus rosa-sinensis and evaluation of antioxidant activity. Foods. 2023; 12(5): 963.
27. Barde SR, Jadhav RS. Hibiscus rosa-sinensis as flavoring agent for alcoholic beverages: a study on antioxidant activity and phenolic content. ResGate. 2021.
28. Zulkurnain EI, Ramli S, Ali AA, James RJ, Kamarazaman IS, Halim H. Phytochemical and pharmacological effects of Hibiscus rosa-sinensis: a review. Int J Pharm Investig. 2023;13(3): 422-31.
29. Carcea M, Narducci A, Turfani R, Vallega A. Functional, antioxidant, antibacterial, and antifungal activity of edible flowers. Plants. 2024;13(2): 344.
30. Sharma Y, Sharma R. Pharmacognostical and phytochemical profiling of Hibiscus rosa-sinensis: unveiling the healing potentials of a botanical treasure. 2023.
31. Geeganage T, Gunathilaka U. Hibiscus rosa-sinensis: a traditional herb with modern biomedical insights. Curr Tradit Med. 2024;10(3):198-210.
32. Falade OS, Aderogba MA, Kehinde O, Akinpelu BA, Oyedapo BO, Adewusi SR. Studies on the chemical constituents, antioxidants and membrane stability activities of Hibiscus rosa sinensis. Niger J Nat Prod Med. 2009; 13:58-64.
33. Munir A, Costa RM, Silva JM. Antimicrobial activity of Hibiscus rosa-sinensis extracts against common pathogens. Braz J Pharmacogn. 2025; 35(1): 25-32.
34. Rani S, et al. Antidiabetic effects of Hibiscus rosa-sinensis in streptozotocin-induced diabetic rats. J Pharm Bioallied Sci. 2024; 16(2): 142-7.
35. Amtaghri S, Amssayef A, Slaoui M, Eddouks M. Antihypertensive and vasorelaxant effects of Hibiscus rosa-sinensis through angiotensin-converting enzyme-2 (ACE-2), and Ca2+ channels pathways. Cardiovasc Hematol Disord Drug Targets. 2022; 22(1): 27-37.
36. Aziz MHA, Raduan SZ, Roslida AH, Zakaria ZA, Zuraini A, Hakim MN. Antipyretic activity of two varieties of Hibiscus rosa-sinensis L. Biomed Pharmacol J. 2021; 14(1).
37. Nayak SB, et al. Evaluation of wound healing activity of Hibiscus rosa-sinensis Linn in albino Wistar rats. Int J Mol Med Adv Sci. 2012; 8(3): 101-6.
38. Chang YC, Hsu CC. Antiproliferative and apoptotic effects of Hibiscus rosa-sinensis extracts in human cancer cells. Evid Based Complement Alternat Med. 2014; 2014: 5198834.
39. Shewale PB, et al. Antidepressant-like activity of anthocyanidins from Hibiscus rosa-sinensis flowers in tail suspension test and forced swim test. Indian J Pharmacol. 2012; 44(4): 454-7. doi:10.4103/0253-7613.99303.
40. Vasudeva N, Sharma SK. Post-coital antifertility activity of Hibiscus rosa-sinensis Linn roots. Evid Based Complement Alternat Med. 2008; 5(1): 91-4. doi:10.1093/ecam/nem003.
41. Mohana P, et al. Inhibition of virulence associated traits by β-sitosterol isolated from Hibiscus rosa-sinensis flowers against Candida albicans: mechanistic insight and molecular docking studies. J Microbiol. 2024; 62(12): 1165-75. doi:10.1007/s12275-024-00174-5.
42. Chakraborty A, Bhattacharjee A, Mondal B, Chakraborty M, Mukhopadhyay G. 5α-reductase inhibitory potential of Hibiscus rosa-sinensis: effective for the management of alopecia. 2023: 467-74.
43. Missoum A. An update review on Hibiscus rosa-sinensis phytochemistry and medicinal uses. J Ayurvedic Herb Med. 2018; 4(3): 135-46.
44. Patel M, Sharma A. Anti-inflammatory and uric acid lowering effects of Hibiscus rosa-sinensis. Animals. 2022; 12(3):288.
45. Ujianti I, et al. Effect of Roselle flower extract (Hibiscus sabdariffa Linn.) on reducing steatosis and steatohepatitis in vitamin B12 deficiency rat model. Medicina (Kaunas). 2023; 59(6): 1044. doi:10.3390/medicina59061044.
46. Fatema S, et al. Phytochemical and physicochemical properties of Hibiscus rosa-sinensis leaves extract: a comparison between conventional and microwave assisted extraction. Eur J Biomed Pharm Sci. 2018; 5(7).
47. Extraction and phytochemical screening of Hibiscus rosa-sinensis. Int J Pharm Res Appl. 2024; 9(2).
48. Samal PK. Investigation of antiepileptic activity of Hibiscus vitifolius leaves. Asian J Pharm Technol. 2013; 3(2): 63-6.
49. Wijewardana N, et al. Evaluation of physicochemical and antioxidant property of dehydrated Hibiscus rosa-sinensis flower petals and its stability in product preparation. Int J Innov Res Technol. 2015; 2(2).
50. Extraction and phytochemical screening of Hibiscus rosa-sinensis. Int J Pharm Res Appl. 2024; 9(2).
51. Tiwari SB, et al. Comparative phytochemical and anxiolytic screening of ethanolic extracts of leaves and flowers of Hibiscus rosa-sinensis Linn. Eur Chem Bull. 2023; 12(Special Issue 5).
52. Hussain G. Soxhlet extraction principle, working and usage. ResGate. 2023.
53. Suryani, Muna. Analysis of total anthocyanin levels in Hibiscus (Hibiscus rosa-sinensis L.) preparations by differential pH method using UV-Vis spectrophotometry. Jurnal IPA Pembelajaran IPA. 2024.
54. Ramya, et al. Physico-chemical properties of spray dried anthocyanin extract from Hibiscus flowers. Plant Sci Today. 2024.
55. Phytochemical and pharmacological effects of Hibiscus rosa-sinensis: a review. Int J Pharm Investig. 2023.
56. Kumar S, Sahoo R. Qualitative tests for preliminary phytochemical screening: an overview. Int J Pharm Sci Res. 2020; 84(7): 239-45.
57. Banu Z, Qhursheed A, Alekya A, Shirisha B, Mounika BV, Divya B. Phytochemical and pharmacological screening of Cosmos sulphureus, Ruellia simplex and Hibiscus rosa-sinensis flower extracts for antinociceptive activity. Res J Pharm Technol. 2024; 17(7): 3399-404.
58. Shasthree T, Babu PT. Qualitative and quantitative determination of phytochemical contents of Muntingia calabura. ResGate. 2022.
59. Patel H, Nayak A, Patel R. Preliminary phytochemical screening of Citrullus colocynthis (L.) Schrad. ResGate. 2019.
60. Olaposi TO, Akinniyi AA, Adeniji SA. Qualitative phytochemical screening of Tithonia diversifolia aqueous root extract. ResGate. 2024.
61. Ukaoma AA, Uwaezuoke AU, Akinboboye AU. Phytochemical screening and antibacterial evaluation of aqueous extracts of Hibiscus rosa-sinensis leaves against Aeromonas hydrophila. ResGate. 2018.
62. Munir N, Khilji SA, Rasool S, Khalil A, Sajid ZA. Phytochemical analysis, antibacterial, and antitumor potential of Hibiscus rosa-sinensis Linn. Scientifica. 2025; 2025:1-8.