Author(s): Vani Mamillapalli, Ratna Harika Chapala, Tejaswi Komal Sai Sareddu, Latha Sri Kondaveeti, Santhi Pattipati, Padmalatha Khantamneni


DOI: 10.5958/2231-5713.2020.00025.2   

Address: Vani Mamillapalli1*, Ratna Harika Chapala1, Tejaswi Komal Sai Sareddu1, Latha Sri Kondaveeti1, Santhi Pattipati1, Padmalatha Khantamneni2
1Department of Pharmacognosy and Phytochemistry, Vijaya Institute of Pharmaceutical Sciences for Women, Enikepadu-521108, Vijayawada, Krishna (Dt.), Andhra Pradesh, India.
2Department of Pharmacology, Vijaya Institute of Pharmaceutical Sciences for Women, Enikepadu-521108, Vijayawada, Krishna (Dt.), Andhra Pradesh, India.
*Corresponding Author

Published In:   Volume - 10,      Issue - 3,     Year - 2020

The plant Casuarina equisetifolia, commonly called as horse tail belonging to family Casuarinaceae is used traditionally for the treatment of infections, ulcers, cough, diarrhea etc. The plant is a rich source of tannins and flavonoids. In the current study the plant leaf, fruit aqueous and ethanolic extracts were determined for total flavonoid content followed by in vitro anti-inflammatory activity study by HRBC membrane stabilization and protein denaturation assays. The results indicate that highest amount of rutin equivalent flavonoids were present in ethanolic extract of leaf, aqueous and ethanolic extracts of fruit. Fruit extracts exhibited highest % inhibition of lysis of HRBC. Aqueous leaf and fruit extracts exhibited highest inhibition of protein denaturation, The results indicate that further in vivo studies, phytochemical isolation, characterization studies could be conducted for plant extracts.

Cite this article:
Vani Mamillapalli, Ratna Harika Chapala, Tejaswi Komal Sai Sareddu, Latha Sri Kondaveeti, Santhi Pattipati, Padmalatha Khantamneni. Evaluation of Phytochemical and in Vitro Anti-Inflammatory activity of Leaf and Fruit Extracts of Casuarina equisetifolia. Asian J. Pharm. Tech. 2020; 10(3):143-148. doi: 10.5958/2231-5713.2020.00025.2

1. Fransworth NR. Bioactive compounds from plants. John and Wiley Co., 1990.
2. Sukanya SL, Sudisha J, Hariprasad P, Niranjana SR, Prakash HS, Fathima SK. Antimicrobial activity of leaf extracts of Indian medicinal plants against clinical and phytopathogenic bacteria. Afr J Biotechnol. 2009; 8: 6677-82.
3. Doughari JH, El-Mahmood AM, Tyoyina I. Antimicrobial activity of leaf extracts of Senna obtusifolia (L). Afr J Pharm Pharmaco. 2008; 2(1): 7-13.
4. Khalifa AB. Herbs: Nature's Pharmacy. 1st edition, Arab Cultural Center, Casablanca, Morocco, 2004; 286–288.
5. Kuhnau J. The flavonoids. A class of semi-essential food components: Their role in human nutrition. World Rev Nutr Diet. 1976; 24:117–191.
6. Bell L, Lamport DJ, Butler LT, Williams CM. A Review of the cognitive effects observed in humans following acute supplementation with flavonoids, and their associated mechanisms of action. Nutrients. 2015; 7:10290–10306.
7. Francisco J Perez-Cano, and Margarida C. Flavonoids, Inflammation and Immune System. Nutrients. 2016; 8(10): 659.
8. Abdullatif A, Ahmad N and Abed N Azab. Anti-Inflammatory Activity of Natural Products. Molecules, 2016; 21(10): 1321.
9. Joker D. Casuarina equisetifolia L. Danida Forest Seed Center, 2000.
10. Kantheti USK, Kumar DY, Ganinna B, and Nath PK. Casuarina equisetifolia effect as antidiabetic and antihyperlipidemic on streptozocin induced rats with diabetes. Int J Cur Tren Pharm Res. 2014; 2(3): 432-436.
11. Nash R, Thomas P, Waigh R. Casuarine-A very highly oxygenated pyrrolizidine alkaloid. Tet.Lett., 1994; 35(42): 7849-7852.
12. Vijayalakshmi A, Tripura A, Ravichandiran V. Development and evaluation of anti-acne products from Terminalia arjuna bark. Int J Chem Tech Res, 2011; 3: 320-27.
13. Weiner MA. Ethnomedicine in Tonga Econ Bot. 1971; 25(4): 423-450.
14. Whistler, WA. Polynesian Medicinal Plants. Everbest, Hong Kong, 1992.
15. Prajapati ND, Purohit SS, Sharma AK, and Kumar T. Handbook of medicinal plants. Agrobios, Jodhpur, India, 2003.
16. Ahsan MR, Islam KM, Haque ME and Mossaddik MA. In vitro antimicrobial screening and toxicity study of some different medicinal plants. Wolrd J Agri Sci. 2009a; 5:617-621.
17. Thube, S.A., Patil, M.J., Antiacne effect of polyherbal gel formulation in Sprague-Dawley rats. Int J Res Ayurveda Pharm. 2013; 4: 398-401.
18. Aher AN, Pal SC, Patil UK, Yadav SK, Bhattacharya S. Evaluation of analgesic activity of Casuarina equisetifolia Frost (Casuarinaceae). Asian J Chem. 2010; 22(5): 3525-30.
19. Sriram N. Antidiabetic and antihyperlipidemic activity of bark of Casuarina equisetifolia on streptozocin induced diabetic rats. Int J Pharm Sci Rev Res. 2011; 1(1): 4-8.
20. Uday Sasi K, Yoganand Kumar D, Bhargav G, Kedar Nath P. Casuarina equisetifolia effect as Antidiabetic and Antihyperlipidemic on Streptozocin induced rats with diabetes. Int J Cur Trend Pharm Res. 2014; 2(3): 432-436
21. Saranya VTK, Uma Gowrie S. Phytochemicalanalysisand invitro studies on Antibacterial, Antioxidant and Antiinflammatory activites using Casuarina equisetifolia bark extracts. Int J Pharm Pharm Sci. 2018 a; 10(1): 0975-1491.
22. Saranya V T K, Uma Gowrie S. Pharmacological studies: antibacterial, antioxidant, and anti-inflammatory efficacy of Casuarina equisetifolia root extracts. Asian J Pharm Clin Res. 2018 b; 11(8):270-6.
23. Moazzem Hossen SM, Islam J, Shakhawat Hossain SM., Mofizur Rahman M, and Ahmed F. Phytochemical and biological evaluation of MeOH extract of Casuarina equisetifolia (Linn.) leaves. European Journal of Medicinal Plants. 2014; 4(8):927-936.
24. Kishore DV and Rumana R. Spasmolytic activity of Casuarina equisetifolia bark extract. International Journal of Pharmaceutical Sciences and Research. 2014; 3(5):1452-1456.
25. El-Tantawy WH, Mohamed SA, and Abdul Al Haleem EN. Evaluation of biochemical effects of Casuarina equisetifolia extract on gentamicin-induced nephrotoxicity and oxidative stress in rats. J Clin Biochem. Nutr.2013; 53(3):158–165.
26. Evans WC. Treaseand Evans Pharmacognosy. 14th edition, WB Saunders Co. Ltd, London, 1996, 545-546.
27. Wallis TE. Text Book of Pharmacognosy. CBS Publishers, Delhi, India, 2005, 572-575.
28. Khandelwal KR. Practical Pharmacognosy: Techniques and Experiments. 4th edition, Nirali Prakashan, India, 1998.
29. Nabavi S M, Ebrahimzadeh M A, Nabavi S F, and Jafari M. Free radical scavenging activity and antioxidant capacity of Eryngium caucasicum Trautv and Froripia subpinnata. Pharmacology online, 2008; 3:19-25.
30. Chang CC, Yang MH, Wen HM, and Chern JC. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal. 2002; 10:178-182.
31. Gandhudasan R, Thamaraichhelvan A, Babraj S. Antiinflammatory action of Lannea cormandelica by HBRC membrane stabilization. Fitoterapia, 1991; 50(12): 81-83.
32. Mizushima Y and Kobayashi M. Interaction of anti-inflammatory drugs with serum preoteins, especially with some biologically active proteins. J of Pharma Pharmacol.1968; 20:169- 173.
33. Feirrali M, Signormi C, Ciccolili L, and Comporti M. Iron release and membrane damage in erythrocytes exposed to oxidizing agents, phenylhydrazine, devicene and iso- uranil. Biochemistry Journal. 1992; 285: 295–301.
34. Mounnissamy VM, Kavimani S, Balu V, and Drlin QS. Evaluation of antiinflammatory and membrane stabilizing properties of ethanol extract of Canjera rehedi. Iranian Journal of Pharmacology and Therapeutics. 2008; 6: 235–237.
35. Puspal De, Subhradeep S, and Madhumita J M. Study the antioxidant and In vitro Anti-inflammatory activity by membrane stabilization method of Amaranthus gangeticus leaf extract. J Pharmacogn Phytochem. 2017; 6(4): 103-105
36. Ricciotti E, FitzGerald GA. Prostaglandins and inflammation. Arterioscler. Thromb. Vasc. Biol. 2011; 31:986–1000.
37. Opie EL. On the relation of necrosis and inflammation to denaturation of proteins. J Exp Med., 1962; 115:597–608.
38. Nurul I O, Norrizah J S, Asmah A, Nurul A M A, and Nur IR. In vitro xanthine oxidase and albumin denaturation inhibition assay of Barringtonia racemosa L. and total phenolic content analysis for potential anti-inflammatory use in gouty arthritis. J Intercult Ethnopharmacol. 2016; 5(4): 343-349.
39. Kim HP, Son KH, Chang HW, Kang SS. Anti-inflammatory plant flavonoids and cellular action mechanisms. J Pharmacol Sci. 2004; 96: 229-245.
40. Permender R, Hema Ch, Sushila R, Dharmender R, Vikash Kumar, and Kanchan Kohli. Mechanism of Action of Flavonoids as Anti-inflammatory Agents: A Review. Inflamm. Allergy Drug Targets. 2009; 8: 229-235. 
41. Yoo H, Ku SK, Baek YD, Bae JS. Anti-inflammatory effects of rutin on HMGB1-induced inflammatory responses in vitro and in vivo. Inflamm Res. 2014; 63(3):197-206.
42. Guardia T, Rotelli AE, Juarez AO, Pelzer LE. Anti-inflammatory properties of plant flavonoids. Effects of rutin, quercetin and hesperidin on adjuvant arthritis in rat. Farmaco. 2001; 56(9):683-7.

Recomonded Articles:

Author(s): A. K. Meena, Jyoti Meena, Ankush Jadhav, M. M. Padhi

DOI:         Access: Open Access Read More

Author(s): Beedha. Saraswathi, Dr. T. Satyanarayana, K. Mounika, G. Swathi , K. Sravika, M. Mohan Krishna

DOI: 10.5958/2231-5713.2018.00004.1         Access: Open Access Read More

Author(s): G. Baskar, J. Chandhuru, K. Sheraz Fahad, A.S. Praveen

DOI:         Access: Open Access Read More

Author(s): Chaudhari R. D, Girase P. R, Suryawanshi H. P, Pawar S. P

DOI: 10.5958/2231-5713.2018.00025.9         Access: Open Access Read More

Author(s): Debarshi Kar Mahapatra , Ruchi S. Shivhare , Vinod G. Ugale

DOI: 10.5958/2231-5713.2018.00008.9         Access: Open Access Read More

Author(s): Basavaraj, Someswara Rao B, S.V Kulkarni, Pramod Patil , Chetan Surpur

DOI:         Access: Open Access Read More

Author(s): K. Vanitha. M. Venkataswamy, Sanam Niharika, Alluri Ramesh

DOI: 10.5958/2231-5713.2018.00011.9         Access: Open Access Read More

Author(s): Pratiksha Sonawale, Amol Patil, Asmit Kamble, Mangesh Bhutkar

DOI:         Access: Open Access Read More

Author(s): Madan Singh, Prashant Soni, Neeraj Upmanyu , Yogesh Shivhare

DOI:         Access: Open Access Read More

Author(s): Agnes Nuniek Winantari1, Dwi Setyawan, Siswandono, Sundani Nurono Soewandhi

DOI: 10.5958/2231-5713.2017.00006.X         Access: Open Access Read More

Author(s): R.B. Saudagar, S. Samuel

DOI: 10.5958/2231-5713.2016.00019.2         Access: Open Access Read More

Author(s): Kaviyarasi K., Kanimozhi K., Madhanraj P, Panneerselvam A., Ambikapathy V.

DOI:         Access: Open Access Read More

Author(s): D. M. Shinkar, A. N. Patil , R. B. Saudagar

DOI: 10.5958/2231-5713.2017.00011.3         Access: Open Access Read More

Author(s): K. Hemalatha, D. Niranjan Raj, M. Fousia Begam, Sharanya. V.K., K. Girija

DOI: 10.5958/2231-5713.2017.00019.8         Access: Open Access Read More

Author(s): Raut Indrayani D, Gharge Manisha, Dandwde Sonli, Mohite S.K., Magdum C.S.

DOI: 10.5958/2231-5713.2017.00008.3         Access: Open Access Read More

Author(s): Nitin A. Gaikwad, Abhishek S. Pujari , Indrajeet V. Mane, Ganesh B. Vambhurkar, Pravin P. Honmane

DOI: 10.5958/2231-5713.2019.00017.5         Access: Closed Access Read More

Author(s): Prajapati Krishna V., Jain Vinit C., Prajapati Neelam

DOI: 10.5958/2231-5713.2016.00007.6         Access: Open Access Read More

Author(s): Poonam M. Lalwani, S.D. Barhate, M.M. Bari

DOI: 10.5958/2231-5713.2018.00021.1         Access: Open Access Read More

Asian Journal of Pharmacy and Technology (AJPTech.) is an international, peer-reviewed journal, devoted to pharmaceutical sciences...... Read more >>>

RNI: Not Available                     
DOI: 10.5958/2231–5713 

Recent Articles