Author(s):
Maher Al-Absi, Abdu Faisal, Safwan Alagbarri, Mofeed Al- Nowihi
Email(s):
ph.d_hmg@hotmail.com , mahir120@gmail.com
DOI:
10.5958/2231-5713.2021.00010.6 
Address:
Maher Al-Absi1*, Abdu Faisal2, Safwan Alagbarri1, Mofeed Al- Nowihi2
1Faculty of Pharmacy, Sana’a University, Yemen.
2Biology Department, Faculty of Science, Sana’a University, Yemen.
*Corresponding Author
Published In:
Volume - 11,
Issue - 1,
Year - 2021
ABSTRACT:
The present study was aimed to developed and optimized the formulation of ointment base by using factorial design includes composition for treating skin burns and wounds. The composition includes bees wax, seams seed oil, Beta-sitosterol and pomegranate peels extract. Methods: A 23 randomized full factorial design was utilized in the present study. The responses observed in this study were: Hardness, Viscosity and Spreadability (rheological properties. Pareto charts and main effects plots of each response were done to obtain a optimum proportion of ingredients. Results: The Ointment formulation of pomegranate peel extract significantly shows a good result in all the evaluation test parameters such as Hardness, viscosity and Spreadability. A multi-response prediction analysis was done to obtain the combined optimum that was used to formulate the final product with BW: VAS: SO to be 5: 1.5: 60. Therefore it was conclude that the formula run order 6 consider as a optimized formula with the appropriate rheological characteristics. Conclusion: The optimum formula for the ointment base properties were estimated using the statistical a multi-response prediction analysis, Pareto charts and main effects plots of responses. This ointment base with desired rheological properties can be used for for cosmetic and pharmaceutical usages. It can be concluded that the ointment base obtained with optimal rheological properties can be used for healing burns, scars and wounds applications.
Cite this article:
Maher Al-Absi, Abdu Faisal, Safwan Alagbarri, Mofeed Al- Nowihi. Development and Rheological Properties Evaluation of a Burn Healing Ointment Composed of Pomegranate peels and Beta-sitosterol Using Factorial Design. Asian J. Pharm. Tech. 2021; 11(1):59-65. doi: 10.5958/2231-5713.2021.00010.6
Cite(Electronic):
Maher Al-Absi, Abdu Faisal, Safwan Alagbarri, Mofeed Al- Nowihi. Development and Rheological Properties Evaluation of a Burn Healing Ointment Composed of Pomegranate peels and Beta-sitosterol Using Factorial Design. Asian J. Pharm. Tech. 2021; 11(1):59-65. doi: 10.5958/2231-5713.2021.00010.6 Available on: https://ajptonline.com/AbstractView.aspx?PID=2021-11-1-10
REFERENCES:
1. Kokane, D.D., More, R.Y., Kale, M.B., Nehete, M.N., Mehendale, P.C., Gadgoli, C.H., 2009. Evaluation of wound healing activity of root of Mimosa pudica. J. Ethnopharmacol. 124 (2), 311-315
2. Li, Y., Guo, C., Yang, J., Wei, J., Xu, J., Cheng, S., 2006. Evaluation of antioxidant properties of pomegranate peel extract in comparison with pomegranate pulp extract. Food Chem. 96 (2), 254-260
3. Voravuthikunchai, S.P., Sririrak, T., Limsuwan, S., Supawita, T., Iida, T., Honda, T., 2005. Inhibitory effects of active compounds from Punica granatum pericarp on verocytotoxin production by enterohemorrhagic Escherichia coli O157: H7. J. Health Sci. 51 (5), 590-596.
4. Guo, C., Yang, J., Wei, J., Li, Y., Xu, J., Jiang, Y., 2003. Antioxidant activities of peel, pulp and seed fractions of common fruits as determined by FRAP assay. Nutr. Res. 23 (12), 1719-1726
5. “The Ayurvedic Pharmacopoeia of India” first edition part-I volume-II page no; 31 to 33.
6. Dr. K. R. Khandelwal and Dr. Vrunda Sethi “Practical Pharmacognosy Techniques and Experiments” Nirali Prakashan Printed by Yogiraj Printers Page no; 23.1-23.11
7. C. A. Alalor, I. I. Cecilia, P. A. Chukwuemeka, Evaluation of the Antibacterial activity of Herbal ointments formulated with Methanolic extract of Cassia alata. Asian Journal of Biomedical and pharmaceutical Sciences. 2 (2012) 15-19.
8. D. Akanksha, G. Vikas, K. J. Neetesh, S. Shalendra, B. Neelam, K. J. Dinesh Formulation and Evaluation of Neomycin Sulphate Ointment containing Natural Wound Healing Agent Curcuma longa, International Journal of Pharmaceutical Sciences and Drug Research. 1 (2009) 105-118.
9. Mutimer MN, Riffikin C, Hill JA, Marry E, Cyr NG, Glickman. Synthesis of Methylsilyl Derivates of Procaine and Their Diffusion. J. Am. Pharm. Assoc. Sci, Ed, 45, 1956, 212.
10. Ehrlich HP, Hunt TK. Effect of Cortisone and Vitamin A on Wound healing, Ann. Surg. 167, 1968, 324.
11. Manafi A, Kohanteb J, Mehrabani D, Japoni A, Amini M, Naghmachi M, Zaghi AH, Khalili N. Active immunization using exotoxin A confers protection against Pseudomonas aeruginosa infection in a mouse burn model. BMC Microbiol 2009; 9:23.
12. Hazrati M, Mehrabani D, Japoni A, Montasery H, Azarpira N, Hamidian-shirazi AR, Tanideh N. Effect of Honey on Healing of Pseudomonas aeruginosa Infected Burn Wounds in Rat. J Appl Anim Res 2010;37: 161-165.
13. Hosseini SV, Tanideh N, Kohanteb J, Ghodrati Z, Mehrabani D, Yarmohammadi H. Comparison between Alpha and silver sulfadiazine ointments in treatment of Pseudomonas infections in 3rd degree burns. Int J Surg 2007; 5: 23-6.
14. Upadhyay NK, Kumar R, Siddiqui MS, Gupta A. Mechanism of Wound-Healing Activity of Hippophae rhamnoides L. Leaf Extract in Experimental Burns. Evid Based Complement Alternat Med 2011;2011: 659705.
15. Kahkeshani N, Farahanikia B, Mahdaviani P, Abdolghaffari A, Hasanzadeh Gh, Abdollahi M, Khanavi M. Antioxidant and burn healing potential of Galium odoratum extracts. Res Pharm Sci 2013;8: 197-203.
16. Jesus NZT, Falcao HS, Gomes I, Leite TJA, Lima GRM, Barbosa-Filho JM, et al. Tannins, peptic ulcers and related mechanisms. Int J Mol Sci 2012; 13:3203–28;https://doi.org/10.3390/ijms13033203
17. Jurenka J. Therapeutic applications of pomegranate (Punica granatum L.). Altern Med Rev 2008; 13(2):128-44
18. Patwardhan CB, Warude D, Pushpangadan P, Bhatt N. Ayurveda and traditional chinese medicine: a comparative overview. Evid Based Complement Altern Med 2005; 2(4):465-73; https://doi.org/ 10.1093/ecam/neh140
19. Ghudaib KK, Hanna ER, Jawad AH. Effect of ellagic acid on some types of pathogenic bacteria. J Al-Nahrain Univ 2010; 13(2):79-85; https://doi.org/10.22401/JNUS.13.2.09
20. I. Biljana, T. Loftsson, M. A. Hjalmarsdottir and G. M. Asgrimsdottir, Topical Formulation Comprising Fatty Acid Extract from Cod Liver Oil: Development, Evaluation and Stability Studies. Mar. Drugs. 14 (2016) 105-116
21. S. Pattanayak, S. S. Nayack, S. C. Dinda, D. Panda and K.P. Naval, Evaluation of herbal ointments formulated with methanolic extract of Cajanus scarabaeides, J. Pharm. Allied health Sci. 1 (2) (2011) 47-59