Author(s): Gandla. Kumara Swamy, JM Rajendra Kumar, J.V.L.N. Seshagiri Rao

Email(s): Email ID Not Available

DOI: Not Available

Address: Gandla. Kumara Swamy1*, JM Rajendra Kumar2, J.V.L.N. Seshagiri Rao3.
1Research scholar, Department of Pharmaceutical Analysis, Jawaharlal Nehru Technological University Kakinada, Kakinada - 533 003-Andhra Pradesh, India.
2Mylan Laboratories Limited, Plot no 31, 32, 33and34-A, Anrich Industrial Estate, Bollaram, Medak(Dist) 502325, India.
3Srinivasa Rao College of Pharmacy, Pothinamallayyapalem, Madhurawada, Visakhapatnam-500041.A.P.India.

Published In:   Volume - 5,      Issue - 3,     Year - 2015


ABSTRACT:
The simple, accurate and precise, isocratic RP-HPLC method has been developed and validated for simultaneous estimation of Clinidipine and Valsartan in bulk and tablets dosage forms. The chromatographic separation was achieved on an Waters symmetry C18 (150x4.6 Id, 3.5µ) with a composition of Acetonitrile : phosphate buffer pH 3.5 adjusted with orthophosphoric acid (70:30, v/v) as mobile phase; at a flow rate of 1.0 ml/min. PDA detection of separtion was performed at 254 nm. The retention times were 2.33 and 3.55 minutes. for Clinidipine and Valsartan respectively. Calibration curves were linear (r2>0.998) over the concentration range 1.2-6.0 µg/ml for Clinidipine and 10-50µg/ml Valsartan. The method was validated for accuracy, precision, specificity, linearity, and sensitivity. The proposed method was successfully used for quantitative analysis of tablets. No interference from any component of pharmaceutical dosage form was observed. Validation studies revealed that method is specific, rapid, reliable, and reproducible. The high recovery and low relative standard deviation confirm the suitability of the method for routine determination of Clinidipine and Valsartan in bulk drug and tablet dosage form.


Cite this article:
Gandla. Kumara Swamy, JM Rajendra Kumar, J.V.L.N. Seshagiri Rao. A validated stability indicating RP-HPLC method for simultaneous estimation of Clinidipine and Valsartan in bulk and combined tablet dosage forms. Asian J. Pharm. Tech. 5(3): 2015; 165-174.


Recomonded Articles:

Author(s): Hafsa, Asfa, Nuha Rasheed, Abdul Saleem Mohammad

DOI: 10.5958/2231-5713.2017.00001.0         Access: Open Access Read More

Author(s): Juveriya Fatima, Saniya Khan, Nuha Rasheed, Abdul Saleem Mohammad

DOI: 10.5958/2231-5713.2017.00009.5         Access: Open Access Read More

Author(s): Avinash B. Thalkari, Pawan N. Karwa, Chandrakant S. Gawli

DOI: 10.5958/2231-5713.2018.00017.X         Access: Open Access Read More

Author(s): Safa Mohammed Sadiq, Amtul Kareem, Nuha Rasheed, Abdul Saleem Mohammad

DOI: 10.5958/2231-5713.2017.00002.2         Access: Open Access Read More

Author(s): Abhijit Ray

DOI:         Access: Open Access Read More

Author(s): Abhishek K. Sah, Manmohan Singh Jangdey, Sanjay J. Daharwal

DOI:         Access: Open Access Read More

Author(s): Manohar D. Kengar, Rohit S. Howal, Dattatray B. Aundhakar, Amit V. Nikam, Priyajit S. Hasabe

DOI: 10.5958/2231-5713.2019.00010.2         Access: Closed Access Read More

Author(s): Abdul Saleem Mohammad, Swetha Devidi, Nikhat Fatima, Humera Badar, Syeda Saba Sulthana, Mohammad Akthar Sulthana, Nuha Rasheed

DOI:         Access: Open Access Read More

Author(s): Brijesh Kumar Duvey, Rohit Goyel, Bharat Parashar, Denesh Verma, Hitesh Dhameja, Dharmesh Sharma

DOI:         Access: Open Access Read More

Author(s): Vishwas R. Potphode, Amol S. Deshmukh, Vijay R. Mahajan

DOI:         Access: Open Access Read More

Author(s): P. Swathi, K. Rajeswar Dutt, K. N. V Rao, M. Alagar Raja

DOI: 10.5958/2231-5713.2017.00025.3         Access: Open Access Read More

Author(s): Rana Khan, Aizaz Ahmed Khan, Nuha Rasheed, Abdul Saleem Mohammad

DOI: 10.5958/2231-5713.2017.00012.5         Access: Open Access Read More

Author(s): Rohan Patil, Neha Patil, Aniket Patil , S. J. Shid, V.N. Dange, C.S. Magdum, S.K. Mohite

DOI: 10.5958/2231-5713.2016.00026.X         Access: Open Access Read More

Author(s): S. Kathirvel, R. Raju, B. Seethadevi, A. Suneetha, J. Pavani

DOI:         Access: Open Access Read More

Author(s): S. Kumara Swamy, G. Arun, Bethi Srinivas, Agaiah Goud B

DOI: 10.5958/2231-5713.2016.00014.3         Access: Open Access Read More

Author(s): Jangam Payal R, Thombre Nilima A, Gaikwad Pallavi N

DOI: 10.5958/2231-5713.2017.00027.7         Access: Open Access Read More

Author(s): M. Karthika, K. Pramod, K.C. Ajithkumar, U. S. Jijith

DOI: 10.5958/2231-5713.2016.00025.8         Access: Open Access Read More

Author(s): Tentu Nageswara Rao, A. Vijayalakshmi, Karri Apparao, N. Krishnarao

DOI: 10.5958/2231-5713.2017.00024.1         Access: Open Access Read More

Asian Journal of Pharmacy and Technology (AJPTech.) is an international, peer-reviewed journal, devoted to pharmaceutical sciences...... Read more >>>

RNI: Not Available                     
DOI: 10.5958/2231–5713 


Recent Articles




Tags