Author(s):
Hamid Khan, Mushir Ali, Alka Ahuja, Javed Ali
Email(s):
khanhamid770@gmail.com
DOI:
10.5958/2231-5713.2017.00005.8 
Address:
Hamid Khan1*, Mushir Ali2, Alka Ahuja2, Javed Ali2
1JK College of Pharmacy, Bilaspur, CG, India-495001
2Department of Pharmaceutics, Faculty of Pharmacy, Hamdard University, New Delhi, India-110062
*Corresponding Author
Published In:
Volume - 7,
Issue - 1,
Year - 2017
ABSTRACT:
In the presented work the ultra-performance liquid chromatographic/quadrupole time-of-flight mass spectrometric (UPLC/Q-TOF-MS) method has been developed for simultaneous determination of metformin, glimepiride and pioglitazone in human plasma. For identification of drugs, the Q-TOF mass spectrometer was operated in positive ionization mode and quantification was done using the MS/MS transitions at m/z 130.0 to 71.0 for metformin, 491.00 to 352.00 for glimepiride and 357.00 to 134.00 for pioglitazone. The chromatographic separation was achieved on Acquity UPLCTM BEH C18 (100.0 × 2.1 mm, 1.7µm) column using isocratic mobile phase consisting of acetonitrile-2mM ammonium acetate (50:50, v/v) at a flow rate of 0.20 mL/min. The elution of metformin, glimepiride and pioglitazone was occurred at 0.50, 1.40 and 1.22 min, respectively. The calibration curves were linear over the concentration range of 1-1000 ng/mL for all the drugs. The developed method was validated according to ICH guidelines. The method was applied for pharmacokinetic study of drugs in FDC tablets in human plasma.
Cite this article:
Hamid Khan, Mushir Ali, Alka Ahuja, Javed Ali. Validated UPLC/Q-TOF-MS Method for Simultaneous Determination of Metformin, Glimepiride and Pioglitazone in Human Plasma and its Application to Pharmacokinetic Study. Asian J. Pharm. Tech. 2017; 7(1): 27-32. doi: 10.5958/2231-5713.2017.00005.8
Cite(Electronic):
Hamid Khan, Mushir Ali, Alka Ahuja, Javed Ali. Validated UPLC/Q-TOF-MS Method for Simultaneous Determination of Metformin, Glimepiride and Pioglitazone in Human Plasma and its Application to Pharmacokinetic Study. Asian J. Pharm. Tech. 2017; 7(1): 27-32. doi: 10.5958/2231-5713.2017.00005.8 Available on: https://ajptonline.com/AbstractView.aspx?PID=2017-7-1-5