Author(s): J. Madhusudhanan, P. Mohanambal, V. Sowmya

Email(s): jmadhuj2008@gmail.com

DOI: Not Available

Address: J. Madhusudhanan1*, P. Mohanambal2, V. Sowmya2
1Associate Professor and Head, Department of Biotechnology, Shri Andal Alagar College of Engineering, Mamandur 603 111.
1, 2Final Year, Department of Biotechnology, Shri Andal Alagar College of Engineering, Mamandur 603 111.
*Corresponding Author

Published In:   Volume - 3,      Issue - 4,     Year - 2013


ABSTRACT:
Nanoparticles are found to be useful as carrier for gene delivery. In the present study, the most biocompatible-Gold nanoparticles were used for carrying serine protease. i.e. Trypsin. It is sensitive to the protease inhibitors present inside the cells and these proteases on binding with gold nanoparticle, loses its stability but retain its activity. The Gold Nanoparticle along with the protease was characterized by UV-Vis spectroscopy, TEM and FTIR. The interaction was confirmed between Trypsin and Gold Nanoparticles and its size was found between 20-28nm. Further, the activity of Trypsin bound to Nanoparticles and the ability of AuNP’s as carrier were analyzed by invitro studies on E.coli HB101. Trypsin’s activity on intracellular protein and specifically on ALPase enzyme showed a 25-29% decrease in protein content which directs that Trypsin bound to Nanoparticles has found to be active within the cell. These results suggest that, such proteolytic enzyme can be designed with nanoparticle to lyse the defective proteins produced by the cells due to genetic disorder.


Cite this article:
J. Madhusudhanan, P. Mohanambal, V. Sowmya. Delivery of Trypsin in to Cells using Nanoparticle. Asian J. Pharm. Tech. 2013; Vol. 3: Issue 4, Pg 165-169.


Recomonded Articles:

Author(s): Safa Mohammed Sadiq, Amtul Kareem, Nuha Rasheed, Abdul Saleem Mohammad

DOI: 10.5958/2231-5713.2017.00002.2         Access: Open Access Read More

Author(s): Amit Roy, Pushpa Prasad, Nirmala Gupta

DOI:         Access: Open Access Read More

Author(s): Amit Roy, Pushpa Prasad

DOI:         Access: Open Access Read More

Author(s): Madan Singh, Prashant Soni, Neeraj Upmanyu , Yogesh Shivhare

DOI:         Access: Open Access Read More

Author(s): Rajendra Jangde

DOI:         Access: Open Access Read More

Author(s): Rangarajan Narasimhan, Ambilly Mohan

DOI: 10.5958/2231-5713.2017.00007.1         Access: Open Access Read More

Author(s): Kruti M. Patel, Pratik R. Patel

DOI: 10.5958/2231-5713.2017.00036.8         Access: Open Access Read More

Author(s): Kanakam Vijayabhaskar, Goli Venkateshwarlu, J. Bhaskar, K. Srisailam, More Swapna

DOI:         Access: Open Access Read More

Author(s): Tanushree Chatterjee, Pradeep Kumar Sahu, Shilpi Chatterjee, Jai Godheja

DOI:         Access: Open Access Read More

Author(s): Kanchan R. Pagar, Sarika V. Khandbahale

DOI: 10.5958/2231-5713.2019.00023.0         Access: Closed Access Read More

Author(s): Brijesh Kumar Saroj, Shreyash Tripathi, Mohd Yaqub Khan

DOI: 10.5958/2231-5713.2018.00034.X         Access: Closed Access Read More

Author(s): Babli Thakur, Vinay Pandit, Mahendra Singh Ashawat, Pravin Kumar

DOI: 10.5958/2231-5713.2016.00006.4         Access: Open Access Read More

Author(s): G. Sivasankari, S. Boobalan, D. Deepa

DOI: 10.5958/2231-5713.2018.00013.2         Access: Open Access Read More

Author(s): Kajal P. Badhe, R.B. Saudagar

DOI: 10.5958/2231-5713.2016.00008.8         Access: Open Access Read More

Author(s): Mohd. Yaqub Khan, Brijesh Kumar Saroj, Maryada Roy, Irfan Aziz

DOI: 10.5958/2231-5713.2015.00008.2         Access: Open Access Read More

Author(s): Jai Godheja, S. K. Shekhar, D.R. Modi

DOI:         Access: Open Access Read More

Asian Journal of Pharmacy and Technology (AJPTech.) is an international, peer-reviewed journal, devoted to pharmaceutical sciences...... Read more >>>

RNI: Not Available                     
DOI: 10.5958/2231–5713 


Recent Articles




Tags