The prime aim of the study is to notice the flavonoids, phenolic acids and xanthones in five commercial herbal raw materials namely Pterocarpum marsupium Bark, Ormocarpum cochinchinense leaf, Indigofera tinctoria leaf, Terminalia arjuna bark and Terminalia chebula fruit obtained from Fabaceae and Combretacea family used in daily domestic needs to confirm the presence of common antioxidant secondary metabolites in herbal raw materials. Results of the study clearly revealed that these raw materials from Fabaceae and Combretacea family contains flavonoids, phenolic acids and xanthones. The developed simultaneous HPTLC method can be employed for the routine investigations of flavonoids, phenolic acids and xanthones in herbal raw materials. Pterocarpum marsupium Bark, and Ormocarpum cochinchinense leaf, was ascertain the presence of Rutin and reveals 0.006%,0.051% respectively. Gallic acid was only orginate in Pterocarpum marsupium Bark of about 0.113%. Quercetin was found to be 0.061%, 0.239%, 0.336%,0.066% and 0.036% respectively in all tested five extracts. Catechin was found to be 0.203% in Terminalia arjuna bark. Mangiferin was found to be 0.009%. in Indigofera tinctoria leaf.
Cite this article:
Ramasamy Arivukkarasu, Aiyalu Rajasekaran.Fingerprint Analysis of Herbal Raw Materials available in Market Belongs to Combretacea And Fabaceae family by HPTLC technique using antioxidant markers. Asian Journal of Pharmacy and Technology. 2021; 11(2):130-4. doi: 10.52711/2231-5713.2021.00021
Ramasamy Arivukkarasu, Aiyalu Rajasekaran.Fingerprint Analysis of Herbal Raw Materials available in Market Belongs to Combretacea And Fabaceae family by HPTLC technique using antioxidant markers. Asian Journal of Pharmacy and Technology. 2021; 11(2):130-4. doi: 10.52711/2231-5713.2021.00021 Available on: https://ajptonline.com/AbstractView.aspx?PID=2021-11-2-6
1. Pietta, PG. Flavonoids as antioxidants. Journal of Natural Products. 2000; S 63 (7): 1035–42.
2. Krewson CF, and Naghski J. Some physical properties of rutin. Journal of the American Pharmaceutical Association. 1952; 41 (11): 582–7.
3. Wanda C. Reygaert. Green Tea Catechins: Their Use in Treating and Preventing Infectious Diseases BioMed Research International Volume 2018; 1-9.
4. Jiayu Gao, Jiangxia Hu , Dongyi Hu , and Xiao Yang A Role of Gallic Acid in Oxidative Damage Diseases: A Comprehensive Review Natural Product Communications 2019;1–9.
5. Jyotshna Puja Khare and Karuna Shanker. Mangiferin: A review of sources and interventions for biological activities 2016; 42(5):504–514.
6. Yao Li et al Quercetin, Inflammation and Immunity Nutrients 2016; 8: 167.
7. Miao He et al. A review on the Pharmacological effects of vitexin and isovitexin. Fitoterapia 2016; 115: 74-85.
8. Zhao, Zhaohui; Moghadasian and Mohammed H."Chemistry, natural sources, dietary intake and pharmacokinetic properties of ferulic acid: A review". Food Chemistry 2008; 109 (4): 691–702.
9. Kumar, Naresh Pruthi and Vikas. "Potential applications of ferulic acid from natural sources". Biotechnology Reports. 2014; 4: 86–93.
10. 187 Fake Cancer 'Cures' Consumers Should Avoid". U.S. Food and Drug Administration Archived from the original 2018
11. Harish Chandra Andola, Vijay Kant Purohit. High Performance Thin Layer Chromatography (HPTLC): A Modern Analytical tool for Biological Analysis. Nature and Science 2010; 8(10): 58-61.
12. Meier B, Spriano D. Modern HPTLC--a perfect tool for quality control of herbals and their preparations. J AOAC Int. 2010; 93(5):1399-409.
13. Arivukkarasu R, and Rajasekaran A. Detection of flavonoids, phenolic acids and xanthones in commercial herbal formulations by HPTLC technique. Research Journal of Pharmacognosy and Phytochemistry 2015;76 (1):19-24.